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SPF Eggs

Specification --
Quality Standards Conform to the relevant standards stated in SPF Chicken Microbiological Monitoring (GB/T 17999-2008)
Remark "Experimental Animal Production License" which allows for the production of SPF eggs should be provided in order to demonstrate that the facilities and equipment of the plant meet the quality requirements for the production of SPF eggs, its production process and quality assurance system are well-established and its SPF eggs conforming to the relevant requirements stated in SPF Chicken Microbiological Monitoring (GB/T 17999-2008).

Quality standards of SPF eggs    

   
1 Formulated in accordance with: National Standard of the People's Republic of China-SPF Chicken Microbiological Monitoring GB/T17999.1-2008.

     
2 Quality standards of SPF eggs        

2.1  Age requirements for SPF laying hens: 24 to 66-wk-old SPF chicken flocks.        
2.2   Detection of specific pathogenic microorganisms 
2.2.1  No existence of the following specific pathogenic microorganisms (antigen-antibody should be negative)   

Serial number Pathogenic microorganism Detection method
1 Salmonella pullorum SPA、IA、TA
2 Haemophilus paragallinarum CO、SPA、IA、ELISA
3 Pasteurella multocida CO、AGP、IA
4 Mycoplasma gallisepticum SPA、HI、 ELISA
5 Mycoplasma synoviae SPA、HI、 ELISA
6 Avian influenza virus AGP、HI、ELISA、RT-PCR
7 Newcastle disease virus HI、ELISA
8 Infectious bronchitis virus ELISA、SN、AGP、HI
9 Infectious laryngotracheitis virus ELISA、AGP、SN
10 Infectious bursal disease virus AGP、ELISA、SN
11 Lymphoid leukosis virus    ELISA ELISA
12 Reticuloendotheliosis Virus ELISA、AGP
13 Marek's disease virus AGP
14 Chicken infectious anemia virus ELISA、IFA、PCR
15 Avian reovirus  (viral arthritis) AGP、ELISA
16 Avian encephalomyelitis virus ELISA、AGP、EST、SN
17 Group I avian adenovirus AGP
18 Group III avian adenovirus(EDS) HI、ELISA
19 Fowlpox virus CO、AGP
Annotation: SPA-serum plate agglutination test; EST-embryo sensitive test; IA-isolation of pathogen; SN-serum neutralization test
AGP-agar diffusion test; HI-hemagglutination inhibition test; IFA-indirect immunofluorescence assay; ELISA-enzyme-linked immunosorbent assay; TA-tube agglutination test; CO-clinical observation; RT-PCR-reverse transcription - polymerase chainreactor; PCR-polymerase chain reaction.


2.2.2 Ensure that eggs from suppliers do not contain the abovementioned pathogenic microorganisms, and regular monitoring reports of each batch of eggs should be issued.        
2.2.3 The Quality Management Department of our company will conduct re-detection of the relevant projects of SPF eggs. The antigen (or antibody) detected should be negative. If the test result is positive, double sampling is needed, if the result is still positive, the batch of eggs will be rejected and feedback will be given to the Logistics Department to inform the supplier.
2.2.4 Due to the limitation of carrying out some of the above pathogenic microorganisms sampling tests, in the production process we will detect all the allantoic fluid after sampling and mixing. The test result should also be negative. If the result is positive, conduct re-sampling. If the result remains positive, the batch of eggs and semi-finished products should be judged as unqualified and be discarded, feedback will be given to the Logistics Department to inform the supplier.

     
2.3 Physical index      

Item Quality requirements Description of acceptable criteria and treatment measures
Integrity No obvious breakages or cracks Eggs with breakages and cracks should be rejected. If the breakage rate of eggs exceeds 5%, inform the supplier to pay close attentions to the quality control of selection, packing and transportation process before delivery. Timely rectification should be made, otherwise our company has the right to terminate the supply until such problems are solved.
Egg shell Should be homogeneous white with even dystruphic catcinosis and thickness distribution, no existence of eggs with steel shell, sand shell, dappled shell or thin shell Eggs with uneven shell color, steel shell, sand shell and dappled shell are unqualified. Obvious ones should be rejected; the number of eggs with the above problems should not exceed 30% of that of the entire batch, otherwise our company has the right to terminate the supply until such problems are solved.
Egg shape Regular egg-shape Eggs with long shape, eggs without obvious large end and small end, eggs with irregular shape or thick waist should be rejected; the number of eggs with the above problems should not exceed 30% of that of the entire batch, otherwise our company has the right to terminate the supply until such problems are solved.
Egg Weight 50-65g -
Cleanliness Should be clean and unwashed No feces or strains on the surface; should not be washed with disinfectant. Uncleanly eggs should be rejected, the number of rejected eggs should not exceed 30% of that of the entire batch, otherwise our company has the right to terminate the supply until such problems are solved.
Eggs which do not meet the above quality requirements will be rejected and not counted in the number of delivered eggs. Unqualified eggs are forbidden to be put into production or inspection.
Infertile egg Should be fertilized with a high rate of healthy chicks    The sum of infertile rate and development aborted rate should not exceed 30% of the number of qualified eggs.
Development aborted egg Should not be aborted Select some batches of eggs and incubate them; test the rate of healthy chick, not as quality standard but as evaluation basis.


2.4 Management requirements       
2.4.1 Established chicken flocks: use the separate feeding unit as the final unit for the microbiological monitoring of our company. If eggs produced by a chicken flock feeding unit (referred to as the “flock”) pass the microbial detection by the Quality Management Department and the production workshop process is demonstrated to meet the product quality requirements, then the flock becomes the established chicken flock of our company. On one side, test reports of each batch of eggs should include the sampling results of the flock; on the other side, the egg-laying chicken flock (and the unit number of independent feeding) and production date should be explicitly identified on the box or on the large end of the hatching egg. If the flock is in short supply or there is a need for additional flocks, application for inspection should be submitted 30 days earlier, and samples should be provided for microbiological testing and production process validation. Eggs should be produced by the egg-laying chicken flocks that have passed the test and verification of our company, or as unqualified.
2.4.2  storage time and conditions: the hatching egg should be delivered to our company within 4 days after production. Eggs should be stored in SPF chicken house with a temperature of 18-20 ℃ and a humidity of 60-80% before delivery; the cleanliness of the storage environment should be consistent with the health conditions for SPF hatching eggs storage. 
2.4.3 Transportation conditions: ambient temperature in transport should not exceed the critical temperature of hatching egg development (no more than 22 ℃); in the process of transport and handling, eggs should be handled with care to avoid breakages.
     

 
3 Other requirements      
3.1 Each batch of SPF hatching eggs should be enclosed with the latest inspection report.    
3.2 Each batch of SPF hatching eggs should be enclosed with the “Egg Laying Information Table”

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